Second Dicty Amateur Video Analysis (17-May)

** Updated 18-May **

Some videos have been added today (refresh https://scicast.org/dicty), so I took a second look.  I don’t look through microscopes for a living, so this isn’t final.  Times below are the timestamped clock time, which elapses at about 8m per frame. I collected:

  • TeamNum: Team name number stated in the video title
  • TeamName: Team name and/or PI name(s)
  • t_First: time when the first cell entered the finish zone. There is some ambiguity for cells that hang out on the opening.
  • Num_3hr: #cells in the finish zone by the 3hr mark; not sure how Irimia is counting cells that leave after entering
  • Num_5hr: #cells in the finish zone by the 5hr mark (only 4 videos)
  • Notes: salient notes, in quotes

Often there were two mazes and finish lines visible.  In these cases I tried to count both finish lines.  I’m not sure that’s canonical.  Here are the results as I saw them (Changed format to avoid margin clipping; sorted by Num_3hr):

TeamNum,TeamName,t_First,Num_3hr,Num_5hr,Notes
Team 12,Kortholt,1:36,14,,”3 in at 1:36,4 @ 1:40,at least 14 at 3h”
Team 11,Faix,1:28,12,,3 in @ 1:36; I counted at least 12 entering by 3h
Control HL60,,1:42,9,,9+. I can’t get it to hold the last frame to count.
Team 9 HL60,Insall,2:14,5,,5+. Many cells got bored and went back into the maze.
Team 17,Kimmel,1:36,5,,5+. Can’t hold the last frame.
Caffeine Control,,1:56,4,,Last one just in time.
Race2Control,,2:15,2,5,”Lots of cells,lots of movement,esp. outside the maze.”
Racer X Neutrophils,,2:25,2,,Cell entering 2:25 jumps track next frame 2:30. Entered?
Team1,Strassman & Queller,2:38,1.5,3,Video starts at 1:30; 2nd cell just crossing at 3:00
Team 9,Insall,,0,0,”Video starts at 1:30; hard to see,but cells appear disorganized.”
Team18,Kay,4:07,0,1,Cells outside the maze are much faster!
Team5 HL60,Steinckwich-Besancon,,0,,”Only 5-6 cells visible,hardly moving.”
Team 19 HL60,Tschirhart,,0,,17 cells idly drifting around the mazes.
Team 7,Bruce,,0,,Halfway through the maze at 3h.
Team 10,Beta,,0,,No cells visible on the slide.
Team 20,Devreotes,,0,,Only 1 confused cell. Did preliminary videos have the wrong label?
Team 9,Insall,,0,,About halfway through at 3h.
Team 15,Myre,,0,,About halfway through at 3h.
Team14,Muller-Taubenberger,,,,Irimia says cells did not get to the starting line.

So, likely winners:

  • Overall winner (most finishers at 3h): Team 12 (Kortholt) with 14+ cells.  We had an email from Irimia mid-afternoon saying at that point they also thought 12 was the most likely.  Second place is probably Team 11 (Faix) with 12+ cells in. Third is likely to be either the HL60 line from Team 9 (Insall), or Team 17 (Kimmel), both with at least 5 cells in by 3hr.
  • Fastest: Team 11 (Faix) just edges out Teams 12 (Kortholt) and 17 (Kimmel) for the fastest single cell at 1:28. This may turn on the precise definition of finish line.
  • Smartest: Sorry, I don’t have the patience to count wrong turns.

Notes: Neutrophils need to be photographed more often — the fastest ones are simply sprinting too fast to uniquely infer motion between frames.

You can still edit the Fastest and Smartest questions.  Until Irimia announces the winners anyway.   https://scicast.org/dicty.

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Dicty World Race Course 2014

The 2014 Dicty Race Course (shown below with no cells in it) was collaboratively designed by a team at Daniel Irimia’s lab. Some clever lad even thought it would be funny to put a Pacman in there. Good call!

2014 Dicty Race Course

2014 Dicty Race Course

At the end of the race course is a straightaway that is 200 microns in length. This straightaway is used to measure speed. Since the microscope only takes an image every 5 minutes, the straightaway allows for Daniel’s lab to tell exactly how many microns the cell moved during the 5 min between that photo and the previous one. Speed is not measured while a cell is in the course because the cell might have taken a number of turns since the last image was taken. 

First shot of Dicty cell lines on the move

First shot of Dicty cell lines on the move

To start the race, the cells are dropped into the middle of the maze (circle in upper left) and they begin to migrate outward into the maze on the right. Can you tell what the shortest path is?!

Tweet @scicasters and let us know what you think!